Coding

Part:BBa_K3605003:Design

Designed by: Guangzhong Zhang   Group: iGEM20_JNFLS   (2020-10-21)


Bacteria cellulose synthase (Bcs) from Gluconacetobacter xylinus.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 740
    Illegal BglII site found at 1136
    Illegal XhoI site found at 232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3387
    Illegal NgoMIV site found at 4617
    Illegal NgoMIV site found at 4687
    Illegal NgoMIV site found at 5569
    Illegal NgoMIV site found at 5758
    Illegal NgoMIV site found at 8925
    Illegal NgoMIV site found at 8929
    Illegal AgeI site found at 7281
    Illegal AgeI site found at 7558
    Illegal AgeI site found at 7818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6141
    Illegal BsaI.rc site found at 982
    Illegal BsaI.rc site found at 1705


Design Notes

To avoid the recognition sequence of some restriction enzymes, some bases are modified.


Source

it comes from the genomic sequence of Gluconacetobacter xylinus.

References