Coding
Part:BBa_K3605003:Design
Designed by: Guangzhong Zhang Group: iGEM20_JNFLS (2020-10-21)
Bacteria cellulose synthase (Bcs) from Gluconacetobacter xylinus.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 740
Illegal BglII site found at 1136
Illegal XhoI site found at 232 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3387
Illegal NgoMIV site found at 4617
Illegal NgoMIV site found at 4687
Illegal NgoMIV site found at 5569
Illegal NgoMIV site found at 5758
Illegal NgoMIV site found at 8925
Illegal NgoMIV site found at 8929
Illegal AgeI site found at 7281
Illegal AgeI site found at 7558
Illegal AgeI site found at 7818 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6141
Illegal BsaI.rc site found at 982
Illegal BsaI.rc site found at 1705
Design Notes
To avoid the recognition sequence of some restriction enzymes, some bases are modified.
Source
it comes from the genomic sequence of Gluconacetobacter xylinus.